Methods, shared
The lab's working bench protocols, organized for our own future selves and anyone else who finds them useful. Cite or fork freely.
Phenol-chloroform protocol for genomic DNA extraction from fungal tissue, optimized for downstream long-read sequencing applications.
Column-based gDNA extraction (NEB Monarch kit) workflow for fungal tissue, with lab-specific modifications for yield and purity.
Total RNA recovery from individual colonized ectomycorrhizal root tips, enabling tri-partite host-fungus-bacterium transcriptomic analysis from a single tip.
More protocols will be posted here as we develop and document them.